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the corresponding preimmune animal serums (rabbit, mouse and rabbit, respectively)  (Agilent technologies)


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    Structured Review

    Agilent technologies the corresponding preimmune animal serums (rabbit, mouse and rabbit, respectively)
    The Corresponding Preimmune Animal Serums (Rabbit, Mouse And Rabbit, Respectively), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/preimmune+rabbit+serum/pmc02796680-41-19-27
    Average 90 stars, based on 1 article reviews
    the corresponding preimmune animal serums (rabbit, mouse and rabbit, respectively) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Negative Control:

    Article Title: Recombinant human erythropoietin induces intussusceptive microvascular growth in vivo.
    Article Snippet: Acetonefixed cryostat CAM sections (8 mm), treated with 7.5% H2O2 to destroy endogenous peroxidase, were stained with a three-step avidin–biotin immunoperoxidase, as described elsewhere.20 Received 13 September 2003; accepted 6 November 2003; Published online 11 December 2003 Correspondence: Professor D Ribatti, Department of Human Anatomy and Histology, Piazza Giulio Cesare, 11, Policlinico, Bari I-70124, Italy; Fax: þ 39 080 5478310; E-mail: ribatti@anatomia.uniba.it Leukemia (2004) 18, 331–336 & 2004 Nature Publishing Group All rights reserved 0887-6924/04 $25.00 www.nature.com/leu Briefly, incubation with primary antibody and then biotinlabeled swine antirabbit IgG (Dako, Glostrup, Denmark) and avidin–horseradish peroxidase conjugate (Vector Inc., Burlingame, CA, USA) was followed by red staining with a 3-amino-9ethylcarbazole (Sigma Chemical Co., St Louis, MO, USA) solution and counterstained with Gill’s hematoxylin no. 2 (Polysciences Inc., Washington, PA, USA) and was mounted in buffered glycerine. .. A preimmune rabbit serum (Dako) replacing the primary antibody that served as a negative control. ..

    Article Title: Effect of synthetic peptides on osteoblast adhesion.
    Article Snippet: The quality of the early cell/material interactions is responsible for the long-term functional properties of any implanted device.. Accordingly, ‘‘next generation’’ dental/orthopedic biomaterials should be able to promote osteoblast adhesion thus improving the integration process between surgically placed implants and biological tissues.. Recent studies have identified a wide range of biochemical signals that can be exploited to promote adhesion, migration, proliferation and differentiation of cells.

    Article Title: The human melanoma associated protein melanotransferrin promotes endothelial cell migration and angiogenesis in vivo.
    Article Snippet: Roberta Salaa, Wilfred A. Jefferiesb, Brandie Walkerb, Joseph Yangb, Jacqueline Tiongb, S. K. Alex Lawc, Mariella F. Carlevaroa, d, Eddy Di Marcoa, Angelo Vaccae, Ranieri Canceddaa, d, Fiorella Descalzi Canceddaa, f, Domenico Ribatti1)g a Istituto Nazionale per la Ricerca sul Cancro, Centro Biotecnologie Avanzate, Genova/Italy b Biotechnology Laboratory and the Biomedical Research Centre and the Departments of Medical Genetics, Microbiology and Immunology and Zoology, The University of British Columbia, Vancouver, BC/Canada c MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford/UK d Dipartimento di Oncologia, Biologia e Genetica, Universit¡ di Genova, Genova/Italy e Dipartimento di Oncologia e Scienze Biomediche, Universita¡ di Bari, Bari/Italy f Centro di Studio per la Neurofisiologia Cerebrale, Consiglio Nazionale delle Ricerche, Genova/Italy g Dipartimento di Anatomia Umana e Istologia, Universita¡ di Bari, Bari/Italy

    Article Title: HIF Activation and VEGF Overexpression are Coupled with ZO‐1 Up‐phosphorylation in the Brain of Dystrophic MDX Mouse
    Article Snippet: Afterwards, the sections were washed in the same buffer and counterstained with Gill’s hematoxylin number 2 (Polysciences, Warrington, PA, USA), and mounted in buffered glycerin. .. A preimmune rabbit serum (Dako) replacing the primary antibody served as a negative control. ..

    Article Title: Localization of factor VIII-related antigen in the endothelium of the chick embryo chorioallantoic membrane.
    Article Snippet: In this study, by using a polyclonal antibody against factor VIII-related antigen (FVIII-RA), we have examined the expression of FVIII-RA in the blood and lymphatic vessels of the chick embryo chorioallantoic membrane (CAM).. The antibody marked the endothelium of blood and lymphatic vessels starting from day 8 of incubation and the cytoplasm of the allantoic epithelial cells.. The application of this antibody may be useful for quantifying neovascularization in response to various angiogenic stimuli applied to the CAM.

    Incubation:

    Article Title: Matrix Metalloproteinase-9 (MMP-9) Is Synthesized in Neurons of the Human Hippocampus and Is Capable of Degrading the Amyloid-β Peptide (1–40)
    Article Snippet: After several rinses with PBS, the sections were processed with the peroxidase ABC kit according to the manufacturer’s recommendations (Vector Laboratories, Burlingame, CA). .. Negative controls consisted of sections incubated in solutions of preimmune rabbit serum (Dako). ..

    Binding Assay:

    Article Title: Effect of rosiglitazone on liver structure and function in genetically diabetic Akita mice.
    Article Snippet: .. A specific binding to secondary antibodies (Abs) was prevented by incubating sections with 20% preimmune rabbit serum (45 min., room temperature, X0902, DAKO) for the F4/80, Mac-3, ICAM-1 and VCAM-1 stainings or with 20% or 5% pre-immune goat serum (45 min., room temperature, X0907, DAKO) for the 3-NT and CK-19 staining, respectively. .. Primary and secondary Abs were diluted in Tris-NaCl-blocking buffer and incubated at room temperature: primary Abs were rabbit antinitrotyrosine (1/1000, 1 hr, 06-284, Millipore, 25 Billerica, MA, USA), rat anti-mouse F4/80 (1/100, overnight (ON), MCAP497, AbD Serotec, D€usseldorf, Germany), rat anti-mouse Mac-3 (1/50, ON, 553322, Pharmingen, San Diego, CA, USA), goat anti-mouse ICAM-1 (1/250, ON, AF796, R&D Systems, Minneapolis, MN, USA), goat anti-mouse VCAM-1 (1/100, ON, AF643, R&D Systems) or rabbit anti-mouse CK-19 (1/100, ON, ab15463, Abcam, Cambridge, UK) followed by secondary Abs horseradish peroxidase (HRP)-labelled goat anti-rabbit (GAR) Ab (1/100, 45 min., P0448, DAKO) for the 3- NT staining, biotinylated (B) rabbit anti-rat antibody (1/300, 45 min., E0468, DAKO) pre-incubated with 10% pre-immune mouse serum (M5905, Sigma-Aldrich, St. Louis, MO, USA) for F4/80 and Mac-3 antigen visualization, rabbit anti-goat-B antibody (1/300, 45 min., E0466, DAKO) preincubated with 10% pre-immune mouse serum for the ICAM-1 and VCAM-1 stainings, and GAR-B antibody (1/300, 45 min., E0432, DAKO) for the CK-19 staining.

    Staining:

    Article Title: Effect of rosiglitazone on liver structure and function in genetically diabetic Akita mice.
    Article Snippet: .. A specific binding to secondary antibodies (Abs) was prevented by incubating sections with 20% preimmune rabbit serum (45 min., room temperature, X0902, DAKO) for the F4/80, Mac-3, ICAM-1 and VCAM-1 stainings or with 20% or 5% pre-immune goat serum (45 min., room temperature, X0907, DAKO) for the 3-NT and CK-19 staining, respectively. .. Primary and secondary Abs were diluted in Tris-NaCl-blocking buffer and incubated at room temperature: primary Abs were rabbit antinitrotyrosine (1/1000, 1 hr, 06-284, Millipore, 25 Billerica, MA, USA), rat anti-mouse F4/80 (1/100, overnight (ON), MCAP497, AbD Serotec, D€usseldorf, Germany), rat anti-mouse Mac-3 (1/50, ON, 553322, Pharmingen, San Diego, CA, USA), goat anti-mouse ICAM-1 (1/250, ON, AF796, R&D Systems, Minneapolis, MN, USA), goat anti-mouse VCAM-1 (1/100, ON, AF643, R&D Systems) or rabbit anti-mouse CK-19 (1/100, ON, ab15463, Abcam, Cambridge, UK) followed by secondary Abs horseradish peroxidase (HRP)-labelled goat anti-rabbit (GAR) Ab (1/100, 45 min., P0448, DAKO) for the 3- NT staining, biotinylated (B) rabbit anti-rat antibody (1/300, 45 min., E0468, DAKO) pre-incubated with 10% pre-immune mouse serum (M5905, Sigma-Aldrich, St. Louis, MO, USA) for F4/80 and Mac-3 antigen visualization, rabbit anti-goat-B antibody (1/300, 45 min., E0466, DAKO) preincubated with 10% pre-immune mouse serum for the ICAM-1 and VCAM-1 stainings, and GAR-B antibody (1/300, 45 min., E0432, DAKO) for the CK-19 staining.



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    Image Search Results


    I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit preimmune antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.

    Journal: PLoS ONE

    Article Title: Functional Insights into Recombinant TROSPA Protein from Ixodes ricinus

    doi: 10.1371/journal.pone.0076848

    Figure Lengend Snippet: I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit preimmune antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.

    Article Snippet: TROSPA-coated ELISA microplates (prepared as described above) were incubated for 90 min at room temperature with 100 µl/well serially diluted (in PBST) rabbit TROSPA antiserum or rabbit preimmune serum (Eurogentec).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Fluorescence, Binding Assay